Tissue Sample Preparation for In Vivo Rodent Alkaline Comet Assay
نویسندگان
چکیده
منابع مشابه
Recommendations for conducting the in vivo alkaline Comet assay. 4th International Comet Assay Workshop.
The in vivo alkaline single cell gel electrophoresis assay, hereafter the Comet assay, can be used to investigate the genotoxicity of industrial chemicals, biocides, agrochemicals and pharmaceuticals. The major advantages of this assay include the relative ease of application to any tissue of interest, the detection of multiple classes of DNA damage and the generation of data at the level of th...
متن کاملA modified alkaline Comet assay for in vivo detection of oxidative DNA damage in Drosophila melanogaster.
Modifications to the alkaline Comet assay by using lesion-specific endonucleases, such as formamidopyrimidine-DNA glycosylase (FPG) and endonuclease III (ENDOIII, also known as Nth), can detect DNA bases with oxidative damage. This modified assay can be used to assess the genotoxic/carcinogenic potential of environmental chemicals. The goal of this study was to validate the ability of this modi...
متن کاملRecommendations for increasing alkaline comet assay reliability in plants.
In plants, an increasing interest for the comet assay was shown in the last decade. This versatile technique appears to be promising to detect the genotoxic effect of pollutants and to monitor the environment. However, the lack of a standardised protocol and the low throughput of the assay limit its use in plants. The aims of this paper are to identify key factors affecting comet assay performa...
متن کاملOecd Guideline for the Testing of Chemicals in Vivo Mammalian Alkaline Comet Assay Introduction
1. The in vivo alkaline comet (single cell gel electrophoresis) assay (hereafter called simply the comet assay) is used for the detection of DNA strand breaks in cells or nuclei isolated from multiple tissues of animals, usually rodents, that have been exposed to potentially genotoxic material(s). The comet assay has been reviewed and recommendations have been published by various expert groups...
متن کاملEvaluation of in vivo genotoxicity of cypermethrin in Drosophila melanogaster using the alkaline Comet assay.
The single cell gel electrophoresis (SCGE) assay, also known as the Comet assay, is one of the most promising genotoxicity tests developed in recent years to measure and analyse DNA damage in single cells. The present study was undertaken to assess the in vivo genotoxicity of the synthetic pyrethroid cypermethrin in brain ganglia and anterior mid gut of Drosophila melanogaster. Freshly emerged ...
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ژورنال
عنوان ژورنال: Genes and Environment
سال: 2012
ISSN: 1880-7062,1880-7046
DOI: 10.3123/jemsge.34.50